Fibrinogen Clotting and Fibrino-peptide Formation by Staphylocoagulase and the Coagulase-reacting Factor.

Drummond, Margaret C. (Emory University, Atlanta, Ga.) and Morris Tager. Fibrinogen clotting and fibrino-peptide formation by staphylocoagulase and the coagulase-reacting factor. J. Bacteriol. 85:628-635. 1963.-The mechanism of fibrinogen clotting by staphylocoagulase and the coagulase-reacting factor (CRF) was studied from the standpoint of the products of the reaction, and compared with thrombin clotting of fibrinogen. Both clotting principles effect clotting with the release of nonprotein nitrogenous material, which can be resolved into three peptides by Dowex-50 chromatography. On the basis of the sedimentation constants, electrophoretic mobilities, and end-group analyses of these peptides, it appears that the products of fibrinogen clotting by coagulase-CRF and by thrombin are similar.